Introduction Antibiotic resistance is one of the greatest challenges facing modern healthcare. As more bacteria develop resistance to commonly used antibiotics, treatment options dwindle, leading to longer hospital stays, higher medical costs, and increased mortality. Two major factors contributing to this problem are genetic mutations and biofilm formation. But what do these terms really mean […]
Category Archives: Microbiology
Introduction Antibiotic resistance happens when bacteria evolve to withstand drugs that once effectively eliminated or inhibited them. During infection testing—specifically cultures—when microbial growth is detected, the next step is identifying and classifying the organism. Techniques like Gram staining, biochemical tests, and colony morphology, are employed in this identification process. But why is this step so […]
Introduction This SOP “Standard Operating Procedure (SOP) for Blood Culture using Brain Heart Infusion (BHI)Broth” outlines blood culture preparation, inoculation, incubation, and interpretation. Different microbiological media are used in the laboratory to cultivate and grow various microorganisms. These broth media are made from a combination of brain and heart tissue infusions from animals, typically cows […]
Diagnosis of Vibrio cholerae Laboratory diagnosis of Vibrio cholerae; the bacteria responsible for cholera, and identifying the causative species, plays a great role in effective treatment and controlling outbreaks. Here is the breakdown of what happens in the laboratory for Cholera to be confirmed. Sample Collection The first step in diagnosing cholera involves collecting stool […]
Introducing Cholera In a world where science has advanced and healthcare interventions are almost real-time, one wonders why we still hear of outbreaks of Vibrio cholerae. It simply means that people, (and by people I mean down to the locals) are not aware of the relevant information needed to avoid such incidents or at least […]
What you need to Know About Mpox
Introduction to Gram Staining technique: A Cornerstone in Microbiology Gram staining is an important technique in microbiology. It is a staining method that narrows down the classification of bacteria into two broad groups: Gram-positive and Gram-negative. It is named after Hans Christian Gram, a Danish bacteriologist. Bacteria are a large group of microorganisms that cause […]
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